designed to deliver the Purest
NHS-Activated Silver Nanoparticles Conjugation Kit
Description :
Nanodiagnostics NHS-activated Silver Nanoparticle Conjugation Kits have been optimized for high efficiency one-step conjugation of proteins and other primary amine-containing ligands to silver nanoparticles.

The kits contain ready-to-use pre-made mixtures. No activation or manipulation of the silver nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester silver nanoparticles supplied in the kit to generate your conjugate.

The precisely engineered silver surface coat on our NHS ester-activated silver nanoparticles results in high conjugation efficiency and stable conjugates while minimizing non-specific binding in your assay.

Kit Components
  • • 10nm NHS-Activated Silver Nanoparticles (lyophilized)
  • • Protein Resuspension Buffer
  • • Reaction Buffer
  • • Quencher Solution

Features & Benefits
  • • Results in covalently bound ligand and more stable conjugate
  • • Fast and convenient one-step conjugation reaction with no pre-activation requirements
  • • Spacer between the silver nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
  • • Precisely engineering surface coating that reduces non-specific protein binding in biological assays.
60nm NHS-Activated Silver Nanoparticle Conjugation Kit (3 reactions)
This kit contains enough reagents for conjugation of 3 x 10µg of protein.
Specifications
Surface functionality: NHS-ester 
(~ 1 NHS group/nm2) 
(10kDa PEG-spacer between silver 
surface and NHS-group)
Core diameter: 60nm 
Concentration: OD=50 
(when dissolved to a final volume of 100µl)
Particles per ml: ~3.4E+11 
(when dissolved to a final volume of 100µl)
Absorbance (λmax): 425-450nm





CATALOG NUMBER         PACK SIZE
RN-Ag-NHSkit-60-3x10µg 3x10µg
RN-Ag-NHSkit-60-10x10µg 10x10µg
RN-Ag-NHSkit-60-100µg 100µg
60nm NHS-Activated Silver Nanoparticle Conjugation Kit (10 reactions)
This kit contains enough reagents for conjugation of 10 x 10µg of protein.
Specifications
Surface functionality: NHS-ester 
(~ 1 NHS group/nm2) 
(10kDa PEG-spacer between silver 
surface and NHS-group)
Core diameter: 60nm 
Concentration: OD=50 
(when dissolved to a final volume of 100µl)
Particles per ml: ~3.4E+11 
(when dissolved to a final volume of 100µl)
Absorbance (λmax): 425-450nm




60nm NHS-Activated Silver Nanoparticle Conjugation Kit (MIDI Kit)
This kit contains enough reagents for conjugation of 100µg of protein.
Specifications
Surface functionality: NHS-ester 
(~ 1 NHS group/nm2) 
(10kDa PEG-spacer between silver 
surface and NHS-group)
Core diameter: 60nm 
Concentration: OD=50 
(when dissolved to a final volume of 100µl)
Particles per ml: ~3.4E+11 
(when dissolved to a final volume of 100µl)
Absorbance (λmax): 425-450nm



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